食品与发酵工业2017，Vol.43Issue(4)：208-215,8.DOI:10.13995/j.cnki.11-1802/ts.201704033
转基因食品定量检测方法的验证
Verification of the quantitative method for GMO foods
饶红 1韩玥 1郭铮蕾 1徐姗 1李伟 1谷强 1林远辉1
作者信息
- 1. 北京出入境检验检疫局,北京,100026
- 折叠
摘要
Abstract
According to the requirements of Guidance document from the European Network of GMO laboratories (ENGL) of JRC,"GB/T 19495.5-2004 Detection of genetically modified organisms and derived products-Quantitative PCR methods based on nucleic acid" including four typical indexes,DNA extraction,accuracy,repeatability and LOQrel,was verified.After verification,DNA extraction kit was qualified.The trueness was within ± 25％ of the accepted reference value.RSDr ＜ 25.LOQrel was 0.1％ GMO content.The verification on the national standard confirms that the guidance is applicable for method verification in laboratory.关键词
转基因食品检测/定量检测/方法验证Key words
GMO testing in foods/quantitative testing/method verification引用本文复制引用
饶红,韩玥,郭铮蕾,徐姗,李伟,谷强,林远辉..转基因食品定量检测方法的验证[J].食品与发酵工业,2017,43(4):208-215,8.
