食品与发酵工业2017，Vol.43Issue(4)：232-237,6.DOI:10.13995/j.cnki.11-1802/ts.201704037
高效液相色谱法测定牛羊杂碎等肉类中嘌呤及尿酸
Determination of purine and uric acid in cooked cow and sheep offal by high performance liquid
摘要
Abstract
A high performance liquid Chromatography method for the determination of uric acid,guanine,hypoxanthine,xanthine and adenine in meat was established and applied.The detecting method for Chromatography was:Shiseido CAPCELL PAK-C18 (4.6 mm × 250 mm,5 μm) column,column temperature was at 30 ℃.The mobile phase was 7 × 10-3 mol/L KH2PO4-H3PO4 (pH =3.83)at a flow rate of 1.0 mL/min.The UV detector was used and the wavelength was set at 254 nm and the Sample volume was 10 μL.Result:Under the above conditions,good separation was obtained and four kinds of purines and uric acid was showed in the range of 0.05-50 μg/mL;a good linear relation was observed between the concentration and peak area,and all the correlation coefficients are higher than 0.9996,the limit of detection was between 0.010-0.024 g/mL,precision detection of RSD％ was between 0.25％-0.81％,the recovery rate of each component was between 92.0％-105.0％,the precision RSD％ was between6.27％-11.09％.Conclusion:The method was simple,rapid and reliable,had a good separation for each component,and can be used for the analysis and determination of purine and uric acid in meat products.关键词
高效液相色谱(HPLC)/牛羊杂碎/嘌呤/尿酸Key words
HPLC/cooked chopped entrails of cattle and sheep/purine/uric acid引用本文复制引用
王静莹,薄海波,吉生军,程子毓,陈秀红..高效液相色谱法测定牛羊杂碎等肉类中嘌呤及尿酸[J].食品与发酵工业,2017,43(4):232-237,6.基金项目
青藏高原特色风味食品中危害物质高通量筛查及快速检测技术研究(2014-ZJ-722) （2014-ZJ-722）
