现代食品科技2017，Vol.33Issue(4)：67-72,18,7.DOI:10.13982/j.mfst.1673-9078.2017.4.011
沙虫蛋白酶解产物抗氧化与免疫调节活性的研究
Antioxidant and Immunomodulatory Activities of the Protein Hydrolysate from Sipunculus nudus
摘要
Abstract
Enzymatic hydrolysis of Sipunculus nudus was performed using Protamex to obtain SNPH.Subsequently,the amino acid composition of SNPH was analyzed,and the distribution of relative molecular mass was determined using a high performance gel chromatography method.The 2,2-diphenyl-l-picrylhydrazyl (DPPH) free radical scavenging activity,hydroxyl radical scavenging activity,superoxide anion scavenging activity,and reducing power of SNPH were evaluated using ultraviolet (UV) spectrophotometry.The effects of SNPG treatment on the splenic lymphocyte proliferation induced by concanavalin (Con) A and lipopolysaccharide (LPS) in mice were determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.The results indicated that SNPH had high contents of essential amino acids and hydrophobic amino acids.The molecular weight distribution showed that SNPH had a high content of oligopeptides,and the substances with a relative molecular mass of 120～1800 accounted for 85.11％.The antioxidant activity experiment revealed that SNPH possessed potent scavenging activities on DPPH free radical,hydroxyl free radical,and superoxide anion in a good dose-effect relationship.The experiment on immunomodulatory activity indicated that SNPH could have a synergistic effect on the splenic lymphocyte proliferation induced by Con A and LPS.SNPH had good antioxidant and immunomodulatory activities.关键词
沙虫/酶解/抗氧化/免疫调节Key words
Sipunculus nudus/hydrolysis/antioxidant/immunomodulatory引用本文复制引用
孙雪萍,徐艳,刘布鸣,孙振亮,刘新..沙虫蛋白酶解产物抗氧化与免疫调节活性的研究[J].现代食品科技,2017,33(4):67-72,18,7.基金项目
广西壮族自治区海洋生物技术重点实验室开放课题(GLMBT-201407) （GLMBT-201407）
广西中药质量标准研究重点实验室自主研究课题(桂中重系201501) （桂中重系201501）
国家自然科学基金资助项目(41306133) （41306133）
上海市奉贤区科委重点项目(20141001) （20141001）
