现代食品科技2017，Vol.33Issue(4)：160-167,8.DOI:10.13982/j.mfst.1673-9078.2017.4.025
优化营养方式强化蛋白核小球藻生物量及蛋白质和叶绿素生产
Optimization of Trophic Modes for Enhancing the Production of Biomass,Protein, and Chlorophyll from Chlorella pyrenoidosa
摘要
Abstract
The biomass of Chlorella pyrenoidosa and its cellular protein and chlorophyll contents were enhanced by optimizing trophic modes and culture conditions.The results showed that glucose concentration could significantly influence the biomass concentration of C.pyrenoidosa under mixotrophic and heterotrophic conditions (p＜0.05),but no significant effect was found with different nitrate concentrations.The highest biomass concentration (21.3l g/L) was obtained when C.pyrenoidosa was cultivated in heterotrophic culture for four days with medium containing 50 g/L glucose and 3.75 g/L nitrate,and was significantly higher than the highest biomass concentration obtained in mixotrophic culture (14.32 g/L) with a salinity of 15‰ (p＜0.05).After glucose depletion,the heterotrophic C.pyrenoidosa strain was diluted with a nitrate-containing medium without glucose for photon-induced cultivation,and the concentration of nitrate was optimized.The results showed that 48-hour photon-induced cultivation under a low light intensity (6,411±532 Lux) in a medium containing 3.75 g/L nitrate produced high contents of protein (54.1％) and chlorophyll (3.14％) in C.pyrenoidosa;these values were increased by 207.74％ and 342.25％,respectively,compared with those obtained before photon-induced cultivation.Additionally,the yields of protein and chlorophyll reached 1.32 g/(L·d) and 0.09 g/(L·d),respectively,significantly higher than the peak values from mixotrophic or heterotrophic culture (p＜0.05).关键词
营养方式/蛋白核小球藻/生物量浓度/蛋白质/叶绿素Key words
trophic modes/Chlorella pyrenoidosa/biomass concentration/protein/chlorophyll引用本文复制引用
魏东,张会贞,陈娇敏..优化营养方式强化蛋白核小球藻生物量及蛋白质和叶绿素生产[J].现代食品科技,2017,33(4):160-167,8.基金项目
国家863计划项目(2013AA065802) （2013AA065802）
国家自然科学基金项目(31370383) （31370383）
广东省海洋渔业科技与产业发展专项(A201401C01) （A201401C01）
广东省公益研究与能力建设项目(2015A020216003、2016A010105001) （2015A020216003、2016A010105001）
