现代食品科技2017，Vol.33Issue(5)：14-19,6.DOI:10.13982/j.mfst.1673-9078.2017.5.003
麒麟菜多糖体内抗肿瘤机理研究
Study of the Antitumor Mechanism of Eucheuma Polysaccharides In vivo
摘要
Abstract
The mechanism of the inhibitory effect ofEucheuma polysaccharides on the proliferation of H22 hepatocellular carcinoma cells (H22) in mice was investigated in vivo.An animal tumor model was established and changes in tumor size and organ indices of the mice in all groups were observed during the experiments.The content of tumor-specific antibodies in serum were measured via ELISA assay.Meanwhile,the proportions ofT-cell subsets in thymus,spleen,peripheral blood,and solid tumors were detected by flow cytometry,and the cell cycle status of solid tumor cells was analyzed by hematoxylin-eosin staining and propidium iodide single staining.The results showed that administration of 600 mg/(kg·d) Eucheuma polysaccharides could significantly inhibit tumor growth,effectively protect the thymus and spleen,significantly enhance the development of CD4+T cells in the thymus (p＜0.05),and significantly increase the content of tumor-specific antibodies in serum (p＜0.05) and the proportion of T cell subsets in blood (p＜0.05).In addition,the tumor-infiltrating lymphocytes in both the polysaccharide and model groups were mainly CD8+ T cells.However,the apoptosis rate of solid tumor cells in the polysaccharide group was significantly increased (p＜0.05) compared to that of the model group.It was preliminarily concluded that Eucheuma polysaccharides could improve the antitumor effect significantly in tumor-bearing mice and allow CD8+ T cells to effectively inhibit the proliferation of H22 cells with the help of CD4+ T cells and tumor-specific antibodies.关键词
麒麟菜多糖/抗肿瘤/肿瘤特异性抗体/T细胞Key words
polysaccharides from Eucheuma/antitumor/tumor-specific antibody/T cells引用本文复制引用
于娟,纪海玉,王玉芳,贾贺威,刘安军..麒麟菜多糖体内抗肿瘤机理研究[J].现代食品科技,2017,33(5):14-19,6.基金项目
国家自然科学基金面上项目(31271975) （31271975）
