现代食品科技2017，Vol.33Issue(5)：27-32,26,7.DOI:10.13982/j.mfst.1673-9078.2017.5.005
姬松茸多糖ABD的结构表征及抗炎活性研究
Structure Characterization and Anti-inflammatory Activity of Polysaccharide ABD from Agaricus blazei Murill
摘要
Abstract
The structure and anti-inflammatory activity of the water-soluble polysaccharide ABD extracted fiom the fruit body of Agaricus blazei Murill were studied in this paper.After the proteins were removed using the Sevage method,the polysaccharide ABD was obtained by precipitating with 85％ ethanol (final concentration) and purifing using the DEAE-sepharose Fast Flow chromatography column.Analysis of UV full-wavelength spectral scans showed that the ABD polysaccharide did not contain impurities,such as proteins and nucleic acids,and did not have a triple helix structure based on the result of Congo red test.The molecular weight was determined to be 2.058×103 ku by gel-permeation chromatography.The monosaccharide composition of ABD was evaluated by HPLC,and ABD was found to consist of glucose (83.59％),galactose (5.46％),mannose (0.59％),and fucose (1.04％).The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay proved that the ABD polysaccharide had no obvious toxicity to macrophages (RAW264.7) within the concentration range of 62.5～1000 μg/mL.The secretion of nitric oxide and tumor necrosis factor alpha (TNF-a) was determined using a mouse model of lipopolysaccharide-induced inflammation in vitro.The results demonstrated that polysaccharide ABD had a dose-dependent inhibitory effect on the secretion of both nitric oxide and TNF-a,and exhibited excellent anti-inflammatory activity.关键词
姬松茸多糖/巨噬细胞(RAW264.7)/抗炎活性Key words
Agaricus blazei Murill polysaccharide/RAW264.7 macrophages/anti-inflammatory activity引用本文复制引用
刘玮,杨继国,任杰,张登辉,宁正祥..姬松茸多糖ABD的结构表征及抗炎活性研究[J].现代食品科技,2017,33(5):27-32,26,7.基金项目
十二五科技支撑计划子课题(2012BAD33B11) （2012BAD33B11）
