现代食品科技2017，Vol.33Issue(5)：115-120,102,7.DOI:10.13982/j.mfst.1673-9078.2017.5.019
不同培养基及培养条件对单增李斯特菌ERIC-PCR分子分型的影响
Influence of Different Medium Conditions on Molecular Typing of Listeria monocytogenes by Enterobacterial Repetitive Intergenic Consensus PCR
摘要
Abstract
Twenty-six strains ofListeria spp.stored in the laboratory were used as the experimental object here,and the enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) and gel electrophoresis techniques were applied to genotyping.Furthermore,according to the typing results,strains of different subtypes were selected,and the effects of different medium conditions on the results of ERIC-PCR molecular typing of Listeria spp.were explored by changing the culture conditions.The preliminary test results showed that 26 strains of Listeria spp.in the laboratory could be subdivided into at least 11 genotypes,and the number of the strains belonging to genotype Ⅰ was the highest,whereas the numbers of strains belonging to genotypes Ⅲ,Ⅶ,Ⅷ,Ⅸ,Ⅹ,and Ⅺ were the lowest.In addition,the results revealed that the changes in medium and culture conditions affected the typing results of LM89 and LM109 (wild-type Listeria monocytogenes),which belong to the genotypes with the lowest number of strains.From this preliminary study,it can be concluded that different nutritional conditions and culture conditions may have an impact on the genomic stability or gene expression ofListeria spp.Therefore,the host source and culture conditions must be taken into account during basic research on L.monocytogenes pathogenicity and its molecular typing.关键词
培养基/单核细胞增生性李斯特菌/ERIC-PCR/分子分型Key words
medium/Listeria monocytogenes/enterobacterial repetitive intergenic consensus polymerase chain reaction/molecular typing引用本文复制引用
赵晨晖,丁承超,刘箐..不同培养基及培养条件对单增李斯特菌ERIC-PCR分子分型的影响[J].现代食品科技,2017,33(5):115-120,102,7.基金项目
“科技创新行动计划”长三角科技联合攻关领域项目(15395810900) （15395810900）
上海市科委高校能力建设项目(13430502400) （13430502400）
