现代食品科技2017，Vol.33Issue(5)：288-292,310,6.DOI:10.13982/j.mfst.1673-9078.2017.5.046
食源性致病沙门氏菌血清型生物标志物的研究
Study on Biomarkers of Foodborne Pathogenic Salmonella Serotypes
摘要
Abstract
Identifying the characteristic metabolites of foodborne pathogenic Salmonella serotypes is the basis for the research and development of an identification and monitoring technique for foodbome pathogenic microorganisms.In this study,intracellular metabolites were extracted from five different Salmonella serotypes comprising eight standard strains by using metabolomics technology,and intracellular metabolic profile analysis was conducted for each strain.Additionally,fingerprints were constructed and principal component analysis (PCA)was performed.It was found that the metabolites of each serotype had large differences in terms of type and abundance,and some potential characteristic metabolites were found.For example,mercaptoacetic acid and α-hydroxypentanedioic acid were biomarkers of the S.enteritidis serotype;N-acetyl aspartic acid,2(1H)-pyrimidone,and L-threonic acid were only produced by the S.typhi serotype;tetradecanoic acid methyl ester and glyceryl ether-glucoside were released only by S.typhimurium serotype;1,4-naphthoquinone was only produced by S.choleraesuis serotype,and 3-pyridinecarboxylic acid trimethylsilyl ester was unique to S.arizonae serotype.The five Salmonella serotypes could be clearly distinguished by cluster analysis and PCA.As a result,the above specific products could be used as potential biomarkers of Salmonella serotypes.关键词
代谢组学/沙门氏菌/生物标志物/指纹图谱Key words
Metabonomics/Salmonella/Biomarkers/Fingerprint引用本文复制引用
董蓉,吴清平,张菊梅,于泓鹏,马连营,郭伟鹏..食源性致病沙门氏菌血清型生物标志物的研究[J].现代食品科技,2017,33(5):288-292,310,6.基金项目
广东省科技计划项目(2014A040401055、2015B020230006) （2014A040401055、2015B020230006）
