食品工业科技2017，Vol.38Issue(7)：81-85,5.DOI:10.13386/j.issn1002-0306.2017.07.007
铁皮石斛多糖分子量测定及其影响因素分析
Determination of molecular weight of Dendrobium officinale polysaccharides and its influencing factors
摘要
Abstract
Objective:In this study,the molecular weight of the polysaccharide from Dendrobium officinale was detected and its main influence factors were discussed.Method:The polysaccharide of the Dendrobium officinale was obtained through extraction and isolation process.And the purified polysaccharide fraction named WDOPA was obtained using DEAE cellulose and sephadex gel Sephacryl S-300 column.The HPGPC method was performed to determine its molecular weight.The processing method for the polysaccharide from Dendrobium officinale was heating and ultrasonic dealing.The influence factors such as heating temperature,heating time,ultrasonic power and duration time were investigated by single factor.Results:The molecular weight of WDOPA was 776054 u,RSD was within 10％.The heating temperature and time did not have significant effects on the molecular weight of the polysaccharide while the ultrasonic dealing had.As the ultrasonic power and duration time increased,the molecular weight of the polysaccharide decreased.Conclusion:This experimental method could be used for the detection of the molecular weight of the polysaccharide.Ultrasonic dealing would change the molecular weight of the polysaccharide from Dendrobium officinlae,this could be applied in produce polysaccharide with different molecular weight.关键词
超声降解/高效凝胶渗透色谱法/铁皮石斛/多糖/分子量Key words
ultrasonic/High Performance Gel Permeation Chromatography (HPGPC)/Dendrobium officinale/polysaccharide/molecular weight分类
轻工纺织引用本文复制引用
黄俊彬,黄丹丹,陈欢欢,李运容,魏刚..铁皮石斛多糖分子量测定及其影响因素分析[J].食品工业科技,2017,38(7):81-85,5.基金项目
广东省省级科技计划项目(2013B060400022). （2013B060400022）
