食品工业科技2017，Vol.38Issue(7)：105-110,6.DOI:10.13386/j.issn1002-0306.2017.07.012
胁迫因素对玻璃表面蜡样芽孢杆菌菌膜形成的影响分析
Effects of stress factors on the formation of Bacillus cereus biofilm on glass surfaces
摘要
Abstract
Biofilms formed by foodborne pathogenic bacteria Bacillus cereus was used as material,the effect of food processing temperature stress,pH stress,nutritional stress,osmotic stress and preservatives stress on the formation of Bacillus cereus biofilms were investigated,and the formation of the biofilms were measured by ultrasonic plate method and observed crystal violet staining.The results showed that under the temperature stress,the Bacillus cereus biofilm formed at 4 ℃ and 50 ℃,the formation of biofilms were increased then decreased with increasing temperature,Bacillus cereus formed stable biofilms at 30 ℃.Under the pH stress,when the pH was less or more than 7.0,the formation of biofims were significantly reduced,Bacillus cereus formed stable biofilms at pH7.0.Under the stress of nutrition,the abilities of biofilms formation would be improved by adding proper glucose in culture medium and the 4％ glucose had obvious effect on promoting the formation of Bacillus cereus biofilms.Under the osmotic stress,low concentration of sodium chloride promoted the formation of Bacillus cereus biofilms,high concentration of sodium chloride could inhibit the formation of Bacillus cereus biofilms and the 0.5％ sodium chloride had obvious effect on promoting the formation of Bacillus cereus biofilms.Under preservative stress,the Bacillus cereus biofims of adding 0.15％ food preservative were higher than 0.10％.In the food processing,it would not neglect controling bacteria biofilms just only adding food preservatives.关键词
蜡样芽孢杆菌/菌膜/玻璃表面/胁迫因素Key words
Bacillus cereus/biofilm/glass surfaces/stress factors分类
轻工纺织引用本文复制引用
夏俊芳,武运,刘霞,李晓燕,张珍珍,古丽娜孜,马瑶,木丽登,王威,张亚南..胁迫因素对玻璃表面蜡样芽孢杆菌菌膜形成的影响分析[J].食品工业科技,2017,38(7):105-110,6.基金项目
新疆农业大学校前期资助课题(XJAU201522). （XJAU201522）
