食品工业科技2017，Vol.38Issue(7)：111-116,6.DOI:10.13386/j.issn1002-0306.2017.07.013
草酸脱羧酶的修饰及其对草酸钙的吸附研究
Research on the adsorption of the modified oxalate decarboxylase on calcium oxalate
摘要
Abstract
In order to improve the applicability and adsorption property of oxalate decarboxylase (OXDC) on calcium oxalate.EDTAD was used to modify the OXDC,modified site was determined by UPLC-MS,and the adsorption of the enzymes on calcium oxalate(CaOx) were studied and compared.The kinetics and thermodynamics adsorption models were used to fit and analysis the experimental data.Results showed that EDTAD was successfully grafted onto OXDC covalently as an absorption group,the modified sites were located in the α-NH2 of the peptide N side,and the adsorption property of the modified enzyme (EDTAD-OXDC)on calcium oxalate was 53.37％ higher than that of OXDC in initial enzyme concentration of 5 mg/mL,pH7.0,temperature of 37 ℃.Conclusion:which inclicated the stability and adsorption properties of DXDC was improved by grafting with EDTAD.关键词
草酸脱羧酶/乙二胺四乙酸二酐(EDTAD)/修饰/草酸钙结石/吸附Key words
oxalate decarboxylase/EDTAD/modified/calcium oxalate stones/adsorption分类
轻工纺织引用本文复制引用
蔡杏华,巫佳,林日辉,贺俊斌,武怡辰,王心雨..草酸脱羧酶的修饰及其对草酸钙的吸附研究[J].食品工业科技,2017,38(7):111-116,6.基金项目
国家教育部留学回国人员科研启动基金项目(教外司留[2012]1707号) （教外司留[2012]1707号）
广西自然科学基金项目(2014GXNSFAA118045) （2014GXNSFAA118045）
广西高等教育机构人才小高地创新团队计划(GJR201147)资助 （GJR201147）
广西科学研究与技术开发项目(桂科合14125008-1-2) （桂科合14125008-1-2）
2015年广西研究生教育创新计划资助研究生科研创新项目(YCSZ2015129). （YCSZ2015129）
