食品工业科技2017，Vol.38Issue(7)：240-244,5.DOI:10.13386/j.issn1002-0306.2017.07.039
罗勒精油的微波辅助提取工艺及其抑菌效果研究
Microwave-assisted extraction and antibacterial effect of essential oil from Ocimum basilicum Linn
摘要
Abstract
In this study,microwave-assisted extraction was applied to extract the essential oil from Ocimum basilicum Linn with petroleum ether.By using orthogonal methodology,the effects of concentration of NaCl,soaking time,solid-liquid ratio,microwave power and extraction time were investigated.It was found that when soaking time was 6 h,solid-liquid ratio was 1∶ 16,microwave power was 567 W and microwave time was 120 s,the optimal oil yield was 0.3758％ (n =3).Then,essential oil's antibacterial effect was determined by using paper discassay and fold dilution plate method.The results indicated that the minimum inhibitory concentration (MIC)for Escherichia coli,Bacillus subtilis and Staphylococcus aureus were 31.25,62.5,125 μg/mL,respectively,and the minimum bactericidal concentration (MBC) for these were 31.25,125,500 μg/mL,respectively.The antibacterial order was:Escherichia coli ＞ Bacillus subtilis ＞ Staphylococcus aureus.The research provides the theoretical basis for development and comprehensive utilization of Ocimum basilicum Linn.关键词
罗勒/精油/微波辅助提取/抑菌效果Key words
Ocimum basilicum Linn/essential oil/microwave-assisted extraction/antibacterial effect分类
轻工纺织引用本文复制引用
艾薇,刘经伦,李晓娇..罗勒精油的微波辅助提取工艺及其抑菌效果研究[J].食品工业科技,2017,38(7):240-244,5.基金项目
保山学院食品科学重点学科建设项目 （）
云南省教育厅科学研究基金资助性项目(2016ZZX221). （2016ZZX221）
