食品工业科技2017，Vol.38Issue(7)：361-364,4.DOI:10.13386/j.issn1002-0306.2017.07.061
不同食用油脂对大鼠胰岛素抵抗和糖异生的影响
Effect of different oil on insulin resistance and gluconeogenesis in rats
摘要
Abstract
Objective:To compare the effects of different oil on insulin resistance and gluconeogenesis in SD rats.Methods:Male SD rats were divided into four groups(n =7).Rats in the control group were fed normal diet and the high fat diet groups were fed with diet containing different oil(lard,olive,and camellia seeds oil).Rats were fed for 17 weeks.Oral glucose tolerance test (OGTT) and insulin tolerance test (ITT)were performed before sacrificed.The artery blood samples were collected after anesthesia,then serum were separarted to detect the levels of fasting plasma glucose (FPG),fasting serum insulin (FINS) and adiponectin.The mRNA expression of phosphoenolpyruvate carboxykinas (PEPCK)and glucose-6-phosphatase(G6P) related to gluconeogenesis in liver,were analyzed by real time PCR.Results:Rats in each oil-fed group showed different levels of insulin resistance.Lard-fed rats led to the significantly increased in levels of FPG,FINS and HOMA-IR(p ＜ 0.05),impaired OGTTand ITT performance.The level of serum adiponectin was decreased in lard-fed rats compared with others (p ＜ 0.05).The expression of gluconeogenesis-related genes(PEPCK and G6Pase)in all oil-fed rats were higher than those in control group(p ＜0.05).The mRNA expression in lard-fed rats were significantly higher than other groups (p ＜ 0.05).Olive oil andcamellia seeds oil-fed rats showed little difference compared with the normal group,but there was statistical significance(p ＜0.05).Conclusion:Lard riched in saturated fatty acids (SFA)may increase the risk of IR.The olive and camellia seeds oil,which have great amount of unsaturated fatty acids (UFA),induced significantly lower degree of IR than lard (p ＜ 0.05).Camellia seed oil as a characteristic oil in China,can be used as a high quality oil.关键词
食用油/胰岛素抵抗/脂联素/糖异生Key words
oil/insulin resistance/adiponectin/gluconeogenesis分类
轻工纺织引用本文复制引用
卜文婕,李艳,童丽,杨建课,孙敏..不同食用油脂对大鼠胰岛素抵抗和糖异生的影响[J].食品工业科技,2017,38(7):361-364,4.基金项目
青海省重点研发与转化计划项目(2016-SF-126) （2016-SF-126）
国家自然基金面上项目(31670361) （31670361）
皖南医学院重点科研项目培育基金(WK2015Z04). （WK2015Z04）
