食品科学2017，Vol.38Issue(9)：106-112,7.DOI:10.7506/spkx1002-6630-201709017
复合菌系降解黄曲霉毒素B1的效果及组成多样性研究
Degradation of Aflatoxin B1 by a Microbial Consortium and Its Composition and Diversity
摘要
Abstract
External elimination method was applied to obtain an efficient aflatoxin B1-degrading microbial consortium,designated FBAD-2.The degradation rates of 2 000 and 5 000 μg/L AFB1 by the microbial consortium could reach 100％ and 90％ within 120 h,respectively.The toxin degradation ability of FBAD-2 was kept at a high level in the range of 30-70 ℃,with an optimal temperature of 60 ℃.The results of AFB1 detoxification experiment showed that the extracellular enzymes in the supernatant played a major role in the degradation of AFB1.Furthermore,the optimal culture time for enzyme production was 24 h,and the supernatant could completely degrade 5 000 μg/L AFB1 within 48 h.16S rRNA gene clone library analysis showed that FBAD-2 was mainly composed of bacteria from the genera Geobacillus,Symbiobacterium thermopilum,Clostridium and Tepidanaerobacter.关键词
黄曲霉毒素B1/生物降解/复合菌系/微生物组成多样性Key words
aflatoxins B1/degradation/bacterial community/microbial composition and diversity分类
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赵春霞,王轶,程薇,吕育财,龚大春,郭鹏..复合菌系降解黄曲霉毒素B1的效果及组成多样性研究[J].食品科学,2017,38(9):106-112,7.基金项目
公益性行业(农业)科研专项(201303080) （农业）
武汉市科技人才培育计划晨光计划项目(2015070404010189) （2015070404010189）
武汉市第四批“黄鹤英才计划”项目(武人才[2016]1号) （武人才[2016]1号）
湖北省科技支撑计划项目(2015BBA199) （2015BBA199）
湖北省农业科技创新中心创新团队项目(2015-620-007-001) （2015-620-007-001）
