食品科学2017，Vol.38Issue(9)：168-172,5.DOI:10.7506/spkx1002-6630-201709027
原花青素B2代谢产物在小鼠体内组织的分布
Distribution of Procyanidin B2 Metabolites in Mice
摘要
Abstract
In the present study,the metabolite profiles of procyanidin B2 in the plasma,liver,heart,spleen,lung,kidney,brain and small intestine of mice were qualitatively evaluated by high performance liquid chromatography with diode array detector coupled with electrospray ionization ion trap time-of-flight multistage mass spectrometry (HPLC-DAD-ESI-IT-TOF-MSn).The results showed that the major conjugated metabolites included methylation metabolites (8),sulfonation metabolites (7),hydration metabolites (5) and hydroxylation metabolites (1),glucuronidation metabolite (1).Procyanidin B2 was metabolized into 6,8,8,6,3,6,2 and 2 forms in plasma,liver,small intestine,kidney,lung,heart,brain and spleen,respectively;three forms of identified metabolites were found with cleavage of interflavanic bond.The conjugated metabolites of procyanidin B2 were diverse and distributed widely in various tissues of mice.Methylation metabolites,sulfonation metabolites and hydration metabolites were dominant,and the liver was the main organ for the conjugated metabolites of procyanidin B2 according to product formation (Mr ＞ 577.13).The cleavage of interflavanic bond reaction was an important way that procyanidin B2 is absorbed.关键词
原花青素B2/HPLC-DAD-ESI-IT-TOF-MSn技术/代谢产物/组织分布Key words
procyanidin B2/HPLC-DAD-ESI-IT-TOF-MSn/metabolites/tissue distribution分类
轻工纺织引用本文复制引用
肖瀛,尹志婷,胡中志,刘泰驿,周一鸣,周小理..原花青素B2代谢产物在小鼠体内组织的分布[J].食品科学,2017,38(9):168-172,5.基金项目
国家自然科学基金青年科学基金项目(31401504) （31401504）
