食品科学2017，Vol.38Issue(10)：24-30,7.DOI:10.7506/spkx1002-6630-201710005
植物乳杆菌YM-2菌株胞外多糖生物合成工艺优化
Optimization of Biosynthesis Conditions for the Production of Exopolysaccharides by Lactobacillus plantarum YM-2
摘要
Abstract
The fermentation conditions for the production of exopolysaccharides (EPS) by Lactobacillus plantarum YM-2,isolated from traditional fermented tempeh in Yunnan province,were optimized.Firstly,the carbon and nitrogen sources in the medium were screened.Secondly,the effects of carbon source concentration,nitrogen source concentration,culture time and culture temperature on EPS yield were studied through one-factor-at-a-time analysis.Finally,response surface methodology (RSM) based on a four-variable,three-level Box-Behnken design was used to determine the optimal culture conditions as follows:carbon source concentration,30 g/L;nitrogen source concentration,30 g/L;culture time,30.05 h;and culture temperature,36.36 ℃.Under these conditions,the yield of EPS was 257.362 mg/L.关键词
植物乳杆菌/胞外多糖/条件优化/响应面法Key words
Lactobacillus plantarum/exopolysaccharides (EPS)/optimization/response surface methodology (RSM)分类
轻工纺织引用本文复制引用
司天昭,柳陈坚,秦晓萌,李晓然,罗义勇,杨恩..植物乳杆菌YM-2菌株胞外多糖生物合成工艺优化[J].食品科学,2017,38(10):24-30,7.基金项目
国家自然科学基金地区科学基金项目(31560447) （31560447）
