食品科学2017，Vol.38Issue(10)：42-48,7.DOI:10.7506/spkx1002-6630-201710008
蟠桃褐腐病原菌生物学特性及细胞壁降解酶活性
Biological Characteristics and Cell Wall Degrading Enzyme Activities of Flat Peach Brown Rot Pathogen
摘要
Abstract
Brown rot disease caused by Monilinia fructicola is one of the major postharvest diseases of flat peach,which causes serious postharvest loss.Meanwhile,the pathogenicities of M.fructicola for different fruit hosts are different.In this investigation,we studied the biological characteristics of M.fructicola under different culture conditions including temperature,pH,and light,and we also examined the impacts of culture temperature,pH and time on the activities of cell wall degrading enzymes.The results showed that the optimum pH for spore germination and germ tube extension of M.fructicola was 5.0 and it grew well in a weak acidic environment,while the spore germination rate was significantly lower in an excessively acidic environment.The optimum temperature for colony growth was in the range of 20-28 ℃,and the pathogen could not grow normally at a temperature above 36 ℃.Temperature affected colony morphology,and at different temperatures,the colony morphology characteristics were different.Light had little effect on the hyphal growth.The activities of the cell wall degrading enzymes endoglucanase (EG),β-glucosidase (BG),polymethylgalacturonase (PMG),and polygalacturonase (PG) were the highest at 28 ℃.The activities of EG and BG reached the highest values at pH 6.0,while the activities of PMG and PG reached the highest values at pH 7.0.The activities of these four enzymes were the highest after cultivation for 5 days.Regardless of changes in pH,temperature and incubation time,BG activity was higher than EG activity and PG activity was higher than PMG activity.关键词
桃褐腐病原菌/生物学特性/细胞壁降解酶活力/培养条件Key words
Monilinia fructicola/biological characteristics/cell wall degrading enzymes/culture conditions分类
生物科学引用本文复制引用
宋丹丹,孔庆军,李鸿彬,任雪艳..蟠桃褐腐病原菌生物学特性及细胞壁降解酶活性[J].食品科学,2017,38(10):42-48,7.基金项目
国家自然科学基金地区科学基金项目(31260402 （）
31460411 （）
21362028) （）
国家自然科学基金面上项目(31671904) （31671904）
陕西省农业科技创新与攻关项目(2016NY-184 （）
2016NY-194) （）
中央高校基本科研业务费专项(GK201503069 （）
GK201603095) （）
