食品科学2017，Vol.38Issue(10)：49-54,6.DOI:10.7506/spkx1002-6630-201710009
NaClO胁迫下单核细胞增生李斯特菌WaX12及其sigB基因缺失突变株抗氧化胁迫相关功能基因表达比较
Comparative Analysis of the Expression of Antioxidation Related Genes in Listeria monocytogenes WaX12 and Its sigB Gene Deletion Mutant under Sodium Hypochlorite Stress
摘要
Abstract
Objective:The purpose of this study was to explore whether the sigB gene palyed a regulatory role in Listeria monocytogenes (Lm) exposed to sodium hypochlorite stress.Methods:We studied the phenotypic changes and compared the percent increases in reactive oxygen species (ROS) levels in the wild-type Lm WaX12 and its sigB deletion mutant under sodium hypochlorite stress at the median lethal concentration (3 mmol/L).The changes in the transcriptional levels of lmo1433,lmo0906,lmo2344,ohrR and lrno2770 genes were investigated during sodium hypochlorite stress.Results:The wild-type strain presented a stronger tolerance to sodium hypochiorite stress than the mutant,especailly during the stationary growth phase.The percent increase in ROS levels in the wild-type strain was 19.97％ higher than that in the mutant after 30 min of sodium hypochlorite stress,confirming the regulatory role of the sigB gene in Lm under sodium hypochlorite stress.Moreover,the relative expression levels of lmo1433,lmo2344 and ohrR genes in the wild-type strain were much higher than those in the mutant (P ＜ 0.01),as revealed by RT-PCR.No significant differences in the expression levels of lmo0906 and lmo2770 were found between the wild-type and mutant strains.Conclusion:The sigB gene plays an important regulatory role in Lm exposed to NaClO stress,which may be partially achieved by direcdy or indirectly activating some genes regulating the redox balance in the body.关键词
单增李斯特菌/sigB/NaClO/氧化胁迫/RT-PCRKey words
Listeria monocytogenes/sigB/sodium hypochlorite/oxidative stress/RT-PCR分类
轻工纺织引用本文复制引用
周密,唐毓神,王旭,张炜佳,潘迎捷,赵勇..NaClO胁迫下单核细胞增生李斯特菌WaX12及其sigB基因缺失突变株抗氧化胁迫相关功能基因表达比较[J].食品科学,2017,38(10):49-54,6.基金项目
国家自然科学基金面上项目(31271870 （）
31571917) （）
上海市科委计划项目(14DZ1205100 （）
14320502100) （）
上海市科技兴农重点攻关项目(沪农科攻字2014第3-5号 （）
2015第4-8号) （）
上海市科委工程中心建设项目(11DZ2280300) （11DZ2280300）
