食品科学2017，Vol.38Issue(10)：55-60,6.DOI:10.7506/spkx1002-6630-201710010
抗荧光假单胞菌Carnobacterium spp.LB1培养发酵条件的优化
Optimization of Culture Conditions of Carnobacterium spp.LB1 for Enhanced Production of Anti-Pseudomonas fluorescens Metabolites
摘要
Abstract
This study aimed to optimize the culture conditions for Carnobacterium spp.LB1,isolated from the ribbonfish intestine,to produce anti-Pseudomonas fluorescens metabolites.By using one-factor-at-a-time method,three of the five factors were selected for their significant influences on the antibacterial activity.A Box-Behnken design was applied to evaluate the effects of interaction among the chosen variables and optimize them.The optimum culture conditions obtained were as follows:initial pH,6.22;temperature,28.6 C;and glucose concentration,3％,where the percentage inhibition of concentrated fermentation broth harvested after 15 and 18 h against Pseudomonasfluorescens were 80.7％ and 86.3％,respectively.The antibacterial mechanism was due to increased cell wall permeability and leakage of a large amount of alkaline phosphatase (AKP) after the destruction of the cell wall of Pseudomonasfluorescens,leading to metabolic disorders.Finally,the experimental results obtained under the optimized fermentation conditions were in good agreement with the model predictions,indicating that the established model in this study was feasible.关键词
肉食杆菌/荧光假单胞菌/抑菌/响应面Key words
Carnobacterium spp.LB1/Pseudomonas fluorescens/antibacterial activity/response surface methodology分类
生物科学引用本文复制引用
董韩博,谢晶,钱韻芳,杨胜平,苟怡..抗荧光假单胞菌Carnobacterium spp.LB1培养发酵条件的优化[J].食品科学,2017,38(10):55-60,6.基金项目
国家自然科学基金面上项目(31571914) （31571914）
上海市科技兴农重点攻关项目(沪农科攻字(2016)第1-1号) （沪农科攻字(2016）
上海市科委平台能力提升项目(16DZ2280300) （16DZ2280300）
