食品科学2017，Vol.38Issue(10)：149-154,6.DOI:10.7506/spkx1002-6630-201710025
产朊假丝酵母核酸高产株的选育及其发酵性能研究
Breeding of a Candida utilis Mutant for Efficient Nucleic Acid Production and its Fermentation Performance
摘要
Abstract
Candida utilis CL1501 was mutagenized with a combination of UV irradiation and nitrosoguanidine treatment.An excellent mutant with high ability to produce nucleic acid,named as CL15013,was obtained.Its nucleic acid content was 16.8％ on a dry cell weight basis,which was 47.8％ higher than that of CL1501.The dry biomass yield of the mutant was 16.9 g/L in fed-batch culture,which was 94.3％ higher than that from batch culture.Under optimized fed-batch culture conditions obtained by orthogonal array design,the biomass yield was up to 21.3 g/L,which was 144.8％ higher than that from batch cultivation.These results indicated that mutant CL15013 has a great application potential in industrial production.关键词
产朊假丝酵母/复合诱变/核酸/流加培养Key words
Candida utilis/combined mutation/nucleic acid/fed-batch culture分类
化学化工引用本文复制引用
蒋雪薇,岳希洁,陈代文,周志明,罗晓明,叶菁,李晓文..产朊假丝酵母核酸高产株的选育及其发酵性能研究[J].食品科学,2017,38(10):149-154,6.基金项目
清远市科技计划项目(2013A024 （）
2014A023) （）
长沙市科技计划重大专项(kq1601013) （kq1601013）
