食品科学2017，Vol.38Issue(10)：162-167,6.DOI:10.7506/spkx1002-6630-201710027
基于2种衍生化方法对牛乳中寡糖的高效液相色谱-质谱联用分析
Comparative Analysis of Oligosaccharides in Milk Based on Two Different Derivatization Methods by HPLC-Tandem Mass Spectrometry
摘要
Abstract
In the present study,Sep-Pak C18 and porous graphitized carbon column chromatographies were sequentially used to separate and purify oligosacchaddes from defatted and deproteinated milk.After derivatization with phenyl-3-methyl-5-pyrazolone (PMP) or 2-aminoacridone (AMAC),the oligosaccharides were analyzed by high-performance liquid chromatography ion trap time of flight tandem mass spectrometry (HPLC-IT-TOF-MS).AMAC was a better derivatization agent than PMP,since they produced about 67 and 20 chromatographic peaks,respectively.Finally,seven lacto-oligosaccharides were identified under optimized HPLC conditions,and their monosaccharide compositions were determined.关键词
牛乳寡糖/1-苯基-3-甲基-5-唑啉酮/2-氨基吖啶酮/高效液相色谱-串联离子阱飞行时间质谱Key words
milk oligosaccharides/3-methyl-1-phenyl-2-pyrazolin-5-one/2-aminoacridone/high-performance liquid chromatography ion trap time of flight tandem mass spectrometry (HPLC-IT-TOF-MS)分类
化学化工引用本文复制引用
陈新新,芦晶,刘鹭,逄晓阳,张书文,李诚,吕加平..基于2种衍生化方法对牛乳中寡糖的高效液相色谱-质谱联用分析[J].食品科学,2017,38(10):162-167,6.基金项目
国家自然科学基金青年科学基金项目(1401514) （1401514）
“十二五”国家科技支撑计划项目(2013BAD18B10) （2013BAD18B10）
公益性行业(农业)科研专项(201303085) （农业）
