食品与发酵工业2017，Vol.43Issue(5)：64-69,6.DOI:10.13995/j.cnki.11-1802/ts.201705011
福建古田红曲生产用红曲霉菌主要种类的鉴别
Identification of major Monascus sp.involved in industrial production of Gutian Hongqu
摘要
Abstract
To characterize and classify major industrial strains of Monascus sp.originated from Gutian Hongqu,we compared 60 strains selected from our institute's biobank with 5 strains obtained from China Center of Industrial Culture Collection (CICC).The first classification criterion was based upon their colonial morphological characteristics on Wa,CYA and G25 N media.Further characterization was performed by comparing the sequences of rDNA internal transcribed spacer (ITS),28S nuclear ribosomal large subunit rRNA gene (LSU),and 18S nuclear ribosomal small subunit rRNA gene (SSU) of the strains with available sequences in GenBank by BLAST.Neighbor-Joining (N J) tree was constructed according to the ITS sequences.Based upon the morphological characteristics and the nucleotide sequences comparison,the 60 Monascus sp.strains were classified as M.ruber,M.pilosus or M.purpureus.关键词
红曲霉菌/ITS/LSU/SSU/鉴定Key words
Monascus/rDNA internal transcribed spacer (ITS)/28S nuclear ribosomal large subunit rRNA gene (LSU)/18S nuclear ribosomal small subunit rRNA gene (SSU)/identification引用本文复制引用
李志强,刘颖,林风,吴丽云..福建古田红曲生产用红曲霉菌主要种类的鉴别[J].食品与发酵工业,2017,43(5):64-69,6.基金项目
福建省公益类科研院所专项(2015R1009-4) （2015R1009-4）
福建省财政厅省直教育科研专项(闽财指[2015] 1297号) （闽财指[2015] 1297号）
福建省公益类科研院所专项(2014R1009-6) （2014R1009-6）
福州市仓山区科技创新公共服务平台项目(2015K03) （2015K03）
福州市仓山区科技计划项目(2015S05) （2015S05）
