食品与发酵工业2017,Vol.43Issue(5):160-166,7.DOI:10.13995/j.cnki.11-1802/ts.201705026
响应面优化鱼鳔胶原肽制备工艺及其抗氧化活性研究
Optimization of swimming bladder collagen peptide preparation using response surface methodology and its antioxidant activity research
摘要
Abstract
Swim bladder is rich in collagen,high in nutrition,but it is waste and cause environmental pollution due to lack of processing technology,causing severe waste and environmental pollution.The technology parameters of enzymatic hydrolysis swim bladder in preparing collagen peptide through single factor experiment and response surface methodology was optimized,DPPH · scavenging ability was used as the indicator.The antioxidant activities of peptide prepared under the optimal condition were also evaluated.Results indicated that the optimal conditions for preparing antioxidant peptides were:Flavourzyme hydrolyzing for 79.34 min,liquid-to-material of 3.09%,emzyme-collagen ratio of 6 343.43 U/g.Under the above conditions,the DPPH · scavenging ability of swim bladder collagen peptide was 79.98%.The IC50 value for DPPH · and · OH scavenging ability was 8.05 mg/mL and 3.54 mg/mL,respectively.The peptide also exhibited relatively strong reducing power.Therefore,swim bladder is a good source in preparing swim bladder collagen with strong antioxidant activity.关键词
鱼鳔/胶原蛋白/肽/抗氧化活性Key words
swimming bladder/collagen/peptides/antioxidant activity引用本文复制引用
涂宗财,唐平平,郑婷婷,庞娟娟,张露,沙小梅,王辉..响应面优化鱼鳔胶原肽制备工艺及其抗氧化活性研究[J].食品与发酵工业,2017,43(5):160-166,7.基金项目
江西省重大生态安全问题监控协同创新中心资助项目(No,JSX-EW-00) (No,JSX-EW-00)
江西省现代农业产业技术体系建设专项资金资助(No,JXARS-04) (No,JXARS-04)