中国食品学报2017，Vol.17Issue(3)：282-288,7.DOI:10.16429/j.1009-7848.2017.03.035
慕萨莱思酒中原花青素的HPLC测定方法
Determination of Procyanidin from Musalais by High Performance Liquid Chromatography Method
摘要
Abstract
Determination of procyanidin content from Musalais was established using SHIMADZU LC-20A HPLC and diode array detector,with standards such as cyanidin,catechin,epicatechin,and epicatechin-3-gallate.The optimized chromatographic condition was as follow:column with Inertsil DOS-3,5 μm 4.6 ×250 mm;mobile phase A was methanol,and mobile phase B was ultrapure water;proportion of mobile phase:mobile phase A was 35％,and mobile phase B was 65％;flow rate was 1 mL/min;sample volume was 10 μL;column temperature was 38 ℃.Using this HPLC method to detect Musalais,peak effect was well,standard curve was normal,and recoveries were 82％ to 93％.This method had the advantages of simple operation,high accuracy,and short detection time.So it was successfully applied to determine procyanidins form Musalais.关键词
高效液相色谱/慕萨莱思/原花青素/回收率Key words
high performance liquid chromatography (HPLC)/Musalais/procyanidins/recovery引用本文复制引用
王伟华,曹员,莫骏涛,田木星,刘飞..慕萨莱思酒中原花青素的HPLC测定方法[J].中国食品学报,2017,17(3):282-288,7.基金项目
国家自然科学基金项目(31360409,31471667) （31360409,31471667）
