茶叶科学2017，Vol.37Issue(3)：251-257,7.
茶氨酸合成酶基因的SNP挖掘和遗传定位
SNP Detection and Mapping of Theanine Synthetase Gene in Tea Plant
摘要
Abstract
The theanine synthetase (TS) gene is considered as the key functional gene for the synthesis of theanine in tea plant. In this study, an F1 segregating population and its parents were used to detect single nucleotide polymorphism (SNP) in the TS gene for genetic mapping. According to the sequence alignment between the parents, three SNPs were found, and SNP735 was identified to be heterozygous in YS. SNP735 was subsequently transformed into dCAPS marker and used for genotyping in the F1 population. The results showed that the segregation ratio of alleles at this SNP locus was close to 1:1. The dCAPS marker was mapped to LG03 at a position between TM299 and TM517 in the tea genetic map. Meanwhile, significant correlations between the dCAPS marker and the content of theanine and total amino acid were identified in this study.关键词
茶树/TS/SNP/dCAPS标记/定位/茶氨酸Key words
tea plant/TS/SNP/dCAPS marker/mapping/theanine分类
生物科学引用本文复制引用
李小杰,马建强,姚明哲,陈亮..茶氨酸合成酶基因的SNP挖掘和遗传定位[J].茶叶科学,2017,37(3):251-257,7.基金项目
国家茶叶产业技术体系项目(CARS-023)、国家自然科学基金(31170624、31500568) （CARS-023）
