乳业科学与技术2017，Vol.40Issue(2)：23-26,4.DOI:10.15922/j.cnki.jdst.2017.02.005
高效液相色谱法测定发酵乳饮料中果糖、葡萄糖、蔗糖、麦芽糖及乳糖含量
Determination of Fructose, Glucose, Sucrose, Maltose and Lactose in Fermented Milk Drink by High Performance Liquid Chromatography
岳虹 1赵贞 1刘丽君 1李翠枝 1邵建波1
作者信息
- 1. 内蒙古伊利实业集团股份有限公司,内蒙古呼和浩特 010110
- 折叠
摘要
Abstract
A method for the determination of fructose,glucose,sucrose,maltose and lactose in fermented milk drink by high performance liquid chromatography (HPLC) with refractive index detector was developed.The samples were extracted with water.The method was performed on a carbonhydrate column using acetonitdle-water as mobile phase.The average spiked recoveries of fructose,glucose,sucrose,maltose and lactose spiked in the concentration range of 0.2％-10.0％ were between 92.6％ and 102.0％ with relative standard deviation (RSD,n =6) less than 3.00％.The limit of quantitation was 0.2％.The results indicated that this method is accurate,simple,reproducible and suitable for the analysis of fructose,glucose,sucrose,maltose and lactose in fermented milk drink.关键词
高效液相色谱法/发酵乳饮料/果糖/葡萄糖/蔗糖/麦芽糖/乳糖Key words
high performance liquid chromatography (HPLC)/fermented milk drink/fructose/glucose/sucrose/maltose/lactose分类
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岳虹,赵贞,刘丽君,李翠枝,邵建波..高效液相色谱法测定发酵乳饮料中果糖、葡萄糖、蔗糖、麦芽糖及乳糖含量[J].乳业科学与技术,2017,40(2):23-26,4.
