食品工业科技2017，Vol.38Issue(9)：128-132,138,6.DOI:10.13386/j.issn1002-0306.2017.09.016
桃果实褐腐病拮抗菌的筛选、鉴定及其拮抗活性
Isolation and identification of antagonistic bacteria against peach brown rot and its antibacterial activity
摘要
Abstract
Two antagonistic bacteria 12a and 14b were obtained from the peach rhizosphere soil against Monilinia fructicola using serial dilution method and dual-culture inhibition on agar plate.12a and 14b were identified as Bacillus vallismortis and Bacillus altitudinis respectively by phenotypic,physiological,biochemical and 16S rRNA gene sequence analysis.In vitro evaluation of antagonistic activity,the sterile fermentation filtrate and volatile metabolites of strain 12a and 14b both showed a strong inhibition on the mycelia growth of M.fructicola.Especially,the inhibitory effect of the volatile metabolites of strain 12a was more significant(p ＜ 0.05).For further study,in vivo tests indicated that fermentation broth and volafile metabolites of strain 12a and 14b also delayed the onset of brown rot significantly(p ＜ 0.05) inhibited the development of lesion diameter of peach brown rot.关键词
桃褐腐菌/拮抗菌/挥发性代谢产物/无菌发酵滤液/鉴定Key words
Monilinia fructicola/antagonistic bacteria/sterile fermentation filtrate/volatile metabolites/identification分类
轻工纺织引用本文复制引用
殷晓慧,王庆国,张畅,石晶盈..桃果实褐腐病拮抗菌的筛选、鉴定及其拮抗活性[J].食品工业科技,2017,38(9):128-132,138,6.基金项目
山东省科技发展计划项目(2014GNC113009) （2014GNC113009）
“十二五”国家科技支撑计划项目(2015BAD16B03). （2015BAD16B03）
