食品工业科技2017，Vol.38Issue(9)：235-241,7.DOI:10.13386/j.issn1002-0306.2017.09.036
响应面分析法优化黑豆中皂苷的提取工艺及其抗氧化性研究
Optimization of extraction technology by response surface methodology and inoxidizability of saponins from black bean
摘要
Abstract
In this paper,black beans as raw material,the response surface methodology (RSM) was used to optimize the extraction process of saponins from black bean.Based on one-factor-at-a-time experiments,extract yield of the saponins was used as response for establishing a quadratic regression model.The effects of solvent and solvent concentration,solid-liquid ratio,extraction temperature and time,particle size,pH on the yield of saponins from black beans were investigated.The study results showed that the optimum conditions for extraction of saponin from black bean as follows:extraction solvent 80％ ethanol,extraction temperature 80 ℃,solid-liquid ratio 1 ∶ 45 (g/mL),extract for 150 min,particle size 40 mesh of raw materials,pH9,the yield of saponins from black beans was 0.72％.The antioxidant activity study showed that abilities of saponins in black beans to scavenge DPPH and hydroxyl radical and superoxide anion were good and the half-scavenging concentration(IC50)were 0.0506,0.148,0.172 mg/mL respectively.Optimization of the extraction process by RSM of saponins of black bean is convenient and feasible.Saponins of black bean has good antioxidant activity and is worth to develop for application.关键词
黑豆/皂苷/提取工艺/响应面法/抗氧化性Key words
black beans/saponins/extraction technology/response surface/oxidation resistance分类
轻工纺织引用本文复制引用
李霄,薛成虎,弓莹,高立国,李改琴,王二荣..响应面分析法优化黑豆中皂苷的提取工艺及其抗氧化性研究[J].食品工业科技,2017,38(9):235-241,7.基金项目
榆林市科技局计划项目(2014 cxy-01). （2014 cxy-01）
