食品工业科技2017，Vol.38Issue(10)：96-102,7.DOI:10.13386/j.issn1002-0306.2017.10.011
SSR荧光标记技术分析东四省典型绿豆品种的遗传多样性
Genetic diversity of typical mungbean varieties in Northeast China by fluorescent SSR markers
摘要
Abstract
Northeast China is one of the main producing areas of mung bean with abundant resource.It helps to improve the utilization efficiency of breeding that clear the genetic background differences between different mung bean varieties.Capillary electrophoresis detection with fluorescent ssr markers were used to analyze 40 typical mung bean varieties by using 8 SSR molecular markers.A total of 43 alleles were identified with an average of 5.4 alleles per locus.The polymorphic information content(PIC) of tested materials detected by different primers varied from 0.40 to 0.75 with an average of 0.55.The distribution of the average number of allelic variation was Heilongjiang (3.63) =Liaoning (3.63) ＞ Neimenggu (3.50) ＞ Jilin (2.88).The distribution of polymorphic information content (PIC) was Heilongjiang (0.530) ＞ Liaoning (0.527) ＞ Neimenggu (0.469) ＞ Jilin(0.382).The analysis of genetic diversity of mungbean resources from 4 geographic origin indicated that Heilongjiang province was the most diversity region,and the last was Jilin province.Cluster analysis showed that germplasm from Heilongjiang province and Liaoning province were the first group,and those from Inner Mongolia and Jilin province were the second group.The genetic diversity of mung bean varieties in Northeast China was preliminarily identified in this study which provided reference for the recollection and breeding utilization of resources and laid the foundation for the further utilization of mung bean resources.关键词
绿豆/SSR荧光标记/毛细管电泳/遗传多样性Key words
mungbean/SSR fluorescent marker/capillary electrophoresis/genetic diversity分类
轻工纺织引用本文复制引用
赵雅楠,王颖,张东杰..SSR荧光标记技术分析东四省典型绿豆品种的遗传多样性[J].食品工业科技,2017,38(10):96-102,7.基金项目
“十二五”农村领域国家科技计划项目(2012BAD34B02) （2012BAD34B02）
黑龙江省应用技术与开发重大项目(GA14B104) （GA14B104）
大庆科技局创新项目(sjh-2013-5). （sjh-2013-5）
