食品工业科技2017，Vol.38Issue(10)：119-123,128,6.DOI:10.13386/j.issn1002-0306.2017.10.015
刺五加黑木耳酸性多糖化学组成及免疫活性研究
Structural properties and immunostimulatory activity of an acidic polysaccharide from Auricularia auricular-judae cultivated on Eleutherococcus senticosus
摘要
Abstract
An acidic polysaccharide named EAP from Auricularia auricular-judae cultivated on Eleutherococcus senticosus was purified by iron chromatography and gel chromatography.The chemical composition and structure of EAP were analyzed by high performance liquid chromatography(HPLC)and fourier transform infrared spectoscopy(FT-IR).The immunomodulatory effects of EAP on RAW264.7 macrophage were investigated by determining the production of NO and secretion of cytokines (IL-6,IL-10,and TNF-α).The result showed that EAP was an acidic polysaccharide with a molecular weight of 925.9 kDa and aβ configuration.It was composed of mannose,glucose,glucuronic acid,xylose aud galactose in the mole ratio of 64.8:14.7:14.2:3.2:2.0.At 50～250 μg/mL,EAP not only was cytotoxic to RAW264.7 cells,but also could significantly promoted the cell viability(p ＜ 0.01).The secretion of NO and IL-10 induced by EAP at 50 μg/mL was significantly improved (p ＜ 0.01),reached 17.2 μmol/L and 171.5 pg/mL,respectively.Moreover,EPA at 200 μg/mL significantly promoted the secretion of TNF-α and IL-6(p ＜ 0.01).It was concluded that the acidic polysaccharide trom Auricularia auricular-judae cultivated on Eleutherococcus senticosus had good potential of immune enhancement.关键词
酸性多糖/刺五加黑木耳/组成/结构/免疫活性Key words
acid polysaccharide/Auricularia auricular-judae cultivated on Eleutherococcus senticosus/composition/structure/immunostimulatory activity分类
轻工纺织引用本文复制引用
张颖,曾艳,邱广君,郁彭,孙媛霞..刺五加黑木耳酸性多糖化学组成及免疫活性研究[J].食品工业科技,2017,38(10):119-123,128,6.基金项目
863计划-天骄(2013AA102102) （2013AA102102）
天津专项(11ZCZDSY08900). （11ZCZDSY08900）
