食品工业科技2017,Vol.38Issue(10):173-177,184,6.DOI:10.13386/j.issn1002-0306.2017.10.025
内蒙古绒山羊不同部位骨骼肌的转录组分析
The transcriptomic analysis of different skeletal muscles of Cashmere goats
摘要
Abstract
In order to study on the growth rules of the goat and improve the mutton production,it was explored the main signaling pathways and the expression of key genes of longissimus muscle,triceps muscle and glutaeus in Inner Mongolian Cashmere goat.Three skeletal muscles of three cashmere goats were obtained after being slaughtered and total RNA was extracted to establish cDNA library.The Illumina high-throughput sequencing technique and bioinformatics were used to determine transcript abundances and characteristics.The establishment of mRNA level analysis platform by transcription for three skeletal muscles in Cashmere goat received 18.64 Gb of data,92278723 reads of double end.The important genes belong to the myosin light chain and myosin heavy chain family.These results suggested that there were many differences in the muscle transcriptomes between these three skeletal muscles.关键词
绒山羊/骨骼肌/转录组/高通量测序Key words
Cashmere goat/skeletal muscle/transcriptome/Illumina high-throughput sequencing technique分类
轻工纺织引用本文复制引用
赵珺,王乐,苏蕊,张燕军,刘志红,李金泉..内蒙古绒山羊不同部位骨骼肌的转录组分析[J].食品工业科技,2017,38(10):173-177,184,6.基金项目
国家863计划项目(2007AA10Z151) (2007AA10Z151)
国家自然科学基金项目(31260538). (31260538)