食品工业科技2017，Vol.38Issue(10)：211-216,235,7.DOI:10.13386/j.issn1002-0306.2017.10.032
大麦乳酸菌发酵液粉中多酚的提取及其抗氧化性研究
Extraction and antioxidant activity of polyphenols from barley lactobacillus fermented solution
摘要
Abstract
Single factor experiment and an orthogonal array design were used in combination to optimize the extraction conditions of polyphenols from barley Lactobacillus fermented solution (BLFS).High performance liquid chromatography (HPLC) was employed to identify the chemical composition of polyphenols.Antioxidant activities of the extracted polyphenols were also measured in this study.The results showed that under the optimum conditions:ethanol concentration 60％,solid-to-liquid ratio 1∶50 g/mL,extraction temperature 60 ℃,and extraction time 60 min,the extraction yield of BLFS polyphenols was (18.09-± 0.25) mg/g.The results of chromatograph analysis with HPLC showed significant increase of gallic acid,coumaric acid,ferulic acid and rutin contents in barley after lactobacillus fermentation (p ＜ 0.05).On the contray,vanillic acid and p-coumaric acid contents reduced dramatically (p ＜ 0.05).In BLFS polyphenols,main components including rutin,vanillic acid,ferulic acid,coumaric acid,gallic acid,protocatechuic acid and p-coumaric acid,their contents were 35.08,21.28,19.38,11.36,6.80,2.99 μg/g and 0.83 μg/g respectively.The polyphenols extraction from BLFS and barley displayed excellent antioxidant activities with half maximal effective concentration (EC50) values of 0.66 and 0.87,0.88 and 0.96,0.76 and 0.89 mg/mL for scavenging hydroxyl,DPPH radicals and reducing capacity,respectively.Antioxidant activities of BLFS polyphenols extraction were higher than these of barley polyphenols extraction,showed that barley increased antioxidant activity by Lactobacillus Fermentation.关键词
大麦/乳酸菌/抗氧化/多酚/提取/高效液相色谱Key words
barley/lactobacillus/antioxidant activity/polyphenols/extraction/high performance liquid chromatography (HPLC)分类
轻工纺织引用本文复制引用
姚芳,肖香,董英..大麦乳酸菌发酵液粉中多酚的提取及其抗氧化性研究[J].食品工业科技,2017,38(10):211-216,235,7.基金项目
江苏省2013年度普通高校研究生科研创新计划项目(CXZZ13_0694) （CXZZ13_0694）
学院科研课题(NSFYB1304). （NSFYB1304）
