食品工业科技2017，Vol.38Issue(10)：281-285,5.DOI:10.13386/j.issn1002-0306.2017.10.045
香蓼总多酚超声波辅助提取工艺优化及其体外抗氧化活性评价
Optimization of ultrasonic assisted extraction technology of total polyphenol from Polygonum viscosum and its antioxidant activity evaluation in vitro
摘要
Abstract
The extract process of total polyphenol from Polygonum viscosum was optimized and the antioxidant activity of total polyphenol in vitro was evaluated.Ethanol concentration,solid to liquid ratio,and extrction time were chosen as the independent variables using ultrasonic-assisted extration method.The optimum technological parameters of ultrasonic-assisted extraction for total polyphenol from P.viscosum were studied via the orthogonal experiment.Finally,the optimal extraction conditions were obtained:extraction temperature 30 ℃,ethanol concentration was 50％ (v/v),the ratio of solid to liquid was 1∶ 50,ultrasonic time was 25 min,and extraction times were 2 times.It was found that the total polyphenols content from the P.viscosum was (94.6-±0.15) mg/g,average recovery rate of total polyphenol was 100.07％,variation coefficient was 1.00％ (n =5).The antioxidant activity was determined though 1,1-Dipheny 1-2-picrylhydrazyl free radical (DPPH·)scavenging rate and total antioxidant activity test.The results indicated that the antioxidant activity of total polyphenol in P.viscosum was stronger than that in tertiary butylhydroquinone(TBHQ),and the EC50 value (5.5 μg/mL)of DPPH· scavenging rate from Pviscosum was better than the EC50 value (18.0 μg/mL)of TBHQ.Total polyphenol in P.viscosum is a natural antioxidant and free radical scavenger.关键词
香蓼/总多酚/超声波辅助提取/抗氧化活性Key words
Polygonum viscosum/total polyphenol/ultrasonic-assisted extraction/antioxidant activity分类
轻工纺织引用本文复制引用
苟体忠,常飞,王翔..香蓼总多酚超声波辅助提取工艺优化及其体外抗氧化活性评价[J].食品工业科技,2017,38(10):281-285,5.基金项目
国家青年自然科学基金(41303016) （41303016）
贵州省教育厅优秀人才项目(黔教合KY字[2012]099号) （黔教合KY字[2012]099号）
贵州省教育厅“高层次人才引进项目”(院科通[2012]7号) （院科通[2012]7号）
凯里学院分析化学重点学科(院通字[2014] 157号) （院通字[2014] 157号）
贵州省教育质量提升工程项目(黔教合KY字[2014] 228号) （黔教合KY字[2014] 228号）
凯里学院博士启动基金(BS201503). （BS201503）
