食品工业科技2017，Vol.38Issue(10)：296-302,7.DOI:10.13386/j.issn1002-0306.2017.10.048
甘肃甘草多糖的提取、纯化及其生物活性
Extraction, purification and biological activity of polysaccharide from Glycyrrhiza uralcnsis in Gansu
田艳花 1杨兆艳 1刘林凤 1刘娜丽 1郭芸1
作者信息
- 1. 山西药科职业学院食品工程系,山西太原030031
- 折叠
摘要
Abstract
Extraction,purification and biologic al activity of polysaccharide from Glycyrrhiza uralcnsis in Gansu were studied.The optimal ultrasonic-assisted extraction technology of polysaccharide was determined by response surface methodology.The polysaccharide of G.uralensis Fisch.(GCP2) was obtained bv the DEAE-52 anion exchange column chromatography and Sephadex G-100 gel filtration,respectively.The composition and molecular weight of the polysaccharide purified were analyzed by gas chromatography and high performance size exclusion chromatography with multi-angle laser light scattering(HPSEC-LLS).The antioxidant and antibacteria activities of GCP were determined in vitro.Results indicated that the optimal extraction conditions were as follows:extraction temperature 70 ℃,extraction time 85 min,liquid-solid ratio 13∶ 1,ultrasonic power 600 W.Under the optimal extraction conditions,the extraction rate of polysaccharide was up to 4.23％.GCP2 was obtained by the chromatographic technique,which was a yellow white powder and reducing sugar without starch.GCP2 consists of arabinose,glucose and galactose with the molar ratio of 0.166∶ 5.56∶ 1.60,and had the characteristic of glycoconjugate by infrared spectrum analysis.HPSEC-LLS analysis results showed that the GCP2 was composed of three different components of polymer,average molecular weight of the main components was 1.378 × 105 g/mol.GCP2 had strong scavenging effect on · OH free radicals and O2-· free radicals with the IC50 of 3.48 mg/mL and 3.43 mg/mL,and had the reductibility increased with the increase of concentration.Antibacteria test showed that GCP2 had better restraining effect on six different bacteria,especially for Escherichia coli and Pneumonia klebsiella strains.关键词
乌拉尔甘草/多糖/超声提取/响应面分析法/纯化/抗氧化与抑菌活性Key words
Glycyrrhiza uralensis Fisch./polysaccharide/ultrasonic extraction/response surface methodology/purification/antioxidant and antibacteria activity分类
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田艳花,杨兆艳,刘林凤,刘娜丽,郭芸..甘肃甘草多糖的提取、纯化及其生物活性[J].食品工业科技,2017,38(10):296-302,7.
