食品工业科技2017，Vol.38Issue(10)：303-306,317,5.DOI:10.13386/j.issn1002-0306.2017.10.049
啤酒糟多糖提取工艺及其抗肿瘤活性
Extraction and antitumor activity of polysaccharide from beer vinasse
摘要
Abstract
Box-Behnken response surface method was to optimize extraction process of polysaccharide from beer vinasse and the antitumor activity of polysaccharide was evaluated.Based on the single factor experiment,leaching temperature,leaching time and solid liquid ratio as factor,the Box-Behnken central composite method was used to make three factors,three levels experimental design.The polysaccharide yield was the response value to process response surface analysis.Adopting MTT method was inspected the inhibition capability of polysaccharide from beer vinasse at 24,48,72 h to the cell proliferation of HeLa,A549 and HepG2.It turned out that the best process of polysaccharide was when leaching temperature was 96.7 ℃,leaching time was 1.83 h,solid liquid ratio was 1∶27 g/mL,the maximum polysaccharide yield was 6.24％,verifying value was 6.07％,relative error was 2.72％.Polysaccharide of beer vinasse could better inhibit multiplication capacity was 34.85,91.50 and 162.39 μg/mL It showed that the extraction process of polysaccharide from beer vinasse in the study was effective,easy and repeatable.Meanwhile,the polysaccharide could be a potential auxiliary antitumor components to apply in health food and medicine field.关键词
啤酒糟/多糖/响应面法/抗肿瘤Key words
beer vinasse/polysaccharide/response surface method/antitumor分类
轻工纺织引用本文复制引用
姜福佳,翟思羽,孙香盟,马帅,李欣,代云飞,逯家富..啤酒糟多糖提取工艺及其抗肿瘤活性[J].食品工业科技,2017,38(10):303-306,317,5.基金项目
吉林省科技厅重点科技攻关项目(20140204007SF) （20140204007SF）
吉林省产业技术研究与开发专项项目. （）
