中国食品添加剂Issue(5)：80-86,7.
大肠杆菌ubiA过表达对CoQ产量的影响
Oover-expression of ubiA gene in Escherichia coil on the yield of CoQ
摘要
Abstract
The purpose of this study is to obtain a high-yield CoQ producing strain.The recombination of ubiA gene in pkk223-3 plasmid was confirmed by restriction enzyme digestion and sequencing;the strain with recombine plasmid was transformed,the changes of CoQ yield and the types of CoQ were analyzed.The fermentation process was optimized through several key factors:fermentation time,the numbers of pHBA supplements,the fermentation temperature and the amount of dissolved oxygen.The results showed that the strains with ubiA gene were successfully obtained,the yield of CoQ increased 95％ and the types were unchanged;the optimized fermentation conditions were as following:replenishing pHBA 3 times,rotate speed 175 r/min,fermentation temperature 34℃ and fermentation for 15 hours,the CoQ yield achieved 2.08mg/g and increased 216％.In conclusion,it was the first time to use overexpressing ubiA gene by knocking out menA gene,CoQ production of mutant strain is greatly improved with the optimized fermentation conditions.The study goal was achieved.关键词
大肠杆菌/过量表达/ubiA基因/CoQ/发酵工艺Key words
escherichia coli/over-expression/ubiA gene/CoQ/fermentation process分类
医药卫生引用本文复制引用
刘永青,任琳,杨水云,王改芳..大肠杆菌ubiA过表达对CoQ产量的影响[J].中国食品添加剂,2017,(5):80-86,7.基金项目
吕梁学院青年基金项目(ZRQN201509). （ZRQN201509）
