中国食品学报2017，Vol.17Issue(4)：14-20,7.DOI:10.16429/j.1009-7848.2017.04.002
副干酪乳杆菌L9对过敏小鼠淋巴细胞Th1/Th2平衡的调节作用
Regulating Effect of Lactobacillus paracasei L9 on Th1/Th2 Balancing in Allergic Mouse's Splenocytes
摘要
Abstract
Objective:To investigate the effects of Lactobacillus paracasei L9 (L9) on the Th1/Th2 cell balance in splenocytes of bovine β-lactoglobulin (BLG)-sensitized mice and evaluate its efficacy to relieve consequent allergic reactions.Methods:Live/Heat inactived L9 were added to splenocyte from allergic mice and bone marrow dendritic cells (BM-DCs) respectively,then cytokines in the supernatant were detected by ELISA,and CD4+Foxp3 + Treg number in lymphocyte cocultured with L9 induced BMDCs was analyzed by flow cytometry.Results:Live/Heat inactived L9 enhanced IFN-γ but reduced IL-4 productions in the supernatant of splenocytes from allergic mice (P＜ 0.05);Increased significantly the regulatory cytokines production from BM-DCs,such as IL-10 and TGF-β,and increased the number of the CD4+Foxp3+Treg cells in lymphocyte.These results demonstrate that heat L9 balanced the disorder of Thl/Th2 in lymphocyte from allergic mice,increased regulatory cytokines production from BM-DCs,it has the anti-allergy activity.关键词
牛乳蛋白过敏/副干酪乳杆菌L9/树突状细胞/T调节细胞Key words
cow's milk allergy/Lactobacillus paracasei L9/dendritic cell/T regulatory cell引用本文复制引用
杨景,刘松玲,赵亮,任发政..副干酪乳杆菌L9对过敏小鼠淋巴细胞Th1/Th2平衡的调节作用[J].中国食品学报,2017,17(4):14-20,7.基金项目
中央在京高校重大成果转化项目(2012BAD12B08) （2012BAD12B08）
北京市科技计划项目(D141100004814001) （D141100004814001）
