中国食品学报2017，Vol.17Issue(4)：65-71,7.DOI:10.16429/j.1009-7848.2017.04.009
鼠李糖乳杆菌电转化条件的研究
Studies on the Electro-transformation Conditions of Lactobacillus rhamnosus
摘要
Abstract
Objective:Different factors affecting electro-transformation efficiency of Lactobacillus rhamnose were studied and optimal condition of electro-transformation were selected to improve the transformation efficiency.Methods:Lartobacillus rhamnose was used as a host,and the E.coli-lactic acid bacteria shuttle plasmid pMG36e were used as a vector;through the method of protoplast electro-transformation,the conditions needed for transformation efficiency were studied,which included plasmid concentration,electric shock buffer,electro-transformation parameters,composition of the recovery culture and incubation time of recovery.Results:Using 1 μL concentration of 1 μ g/μL plasmids pMG36e,with the electroporation parameters of 8 kV/cm,200 Ω and 25 μF,and proper incubation in MRS broth containing 20 mmol/L MgCl2,CaCI2 and sucrose of 0.5 mol/L (without Twcen 80) for at least 2.5 hours,the best electroporation efficiency was obtained.As a result,the transformational efficiency reaches 1.66×105 CFU/μg DNA.Conclusion:This study has realized efficientgenetic transformation of Lactobacillus rhannosus and provided the technical support for genetic breeding.关键词
鼠李糖乳杆菌/质粒pMG36e/电转化/电转化效率Key words
Lactobacillus rhamnosus/plasmid pMG36e/electro-transformation/transformational efficiency引用本文复制引用
北婷婷,李晨,谷新晰,张会彦,田洪涛,罗云波..鼠李糖乳杆菌电转化条件的研究[J].中国食品学报,2017,17(4):65-71,7.基金项目
国家“863”计划项目(2006AA10Z317) （2006AA10Z317）
河北省自然科学基金项目(C2016204129) （C2016204129）
