中国食品学报2017，Vol.17Issue(4)：98-103,6.DOI:10.16429/j.1009-7848.2017.04.013
甲鱼碱溶蛋白肽的分离及抗肿瘤活性研究
Separation and Antitumor Activity Evaluation of Turtle Alkali-soluble Protein Peptide
摘要
Abstract
In order to obtain peptides with anti-tumor activity.The effects of four proteases (Acid protease,Trypsin,Neutrase,Papain) on degree of hydrolysis of Turtle Alkali-soluble Protein were researched.The results showed Neutrase was the optimal hydrolytic enzyme.Anticancer peptide was produced by hydrolyzing turtle alkali-soluble protein with Neutrase,and was purified with gel chromatography.The molecular weight of high active peptides were determined by HPLC.Results showed the Neutrase hydrolysate with high degree of hydrolysis (DH) was reached to 14.47％.The polypeptide that with 184-4 588 u molecular weight has evident inhibited effects on the growth of human lung cancer A549 cells,its IC50 values was 32.26 μg/mL.关键词
甲鱼碱溶蛋白/肽/A549细胞/抑制作用Key words
Turtle alkali-soluble protein/peptide/A549 cells/growth inhibiting effect引用本文复制引用
王楠,王杰,姜龙达,王伟,陈永洲,沈素芬..甲鱼碱溶蛋白肽的分离及抗肿瘤活性研究[J].中国食品学报,2017,17(4):98-103,6.基金项目
国家自然科学基金青年基金项目(31401500) （31401500）
浙江省自然科学基金一般项目(LY17C200012) （LY17C200012）
国家自然科学基金青年基金项目(31101390) （31101390）
