中国食物与营养2017，Vol.23Issue(4)：56-58,3.
非转基因和转基因小麦中氨基酸含量的分析及比较
Determination and Comparison of Amino Acids in Non-transgenic Wheat and Transgenic Wheat
摘要
Abstract
[Objective]A new method for the determination on 17 amino acids in wheat was established by amino acid analyzer (AAA).The contents of the amino acids in the non-transgenic wheat and the transgenic wheat were compared.[Method] The samples were subjected to hydrolysis by the hydrochloric acid solution containing 0.1％ thioglycolic acid,and were caTied out by AAA.The external standard method was used for quantitative analysis.[Result] This hydrolysis pretreatment process could effectively prevent oxidation of sulfur-containing amino acids (methionine and cysteine).The recoveries were 93％ ～99％,and the relative standard deviations were no more than 2.4 ％.There was no significant difference of the 17 amino acids contents in the non-transgenic wheat and the genetransfer wheat [Conclusion] The method was accurate,and had good repeatability which could meet the requirements for determination of 17 amino acids in wheat.关键词
氨基酸/转基因小麦/非转基因小麦/氨基酸分析仪Key words
amino acid/transgenic wheat/non-transgenic wheat/amino acid analyzer引用本文复制引用
刘婷婷,邢青斌,程家丽,沈葹,卓勤..非转基因和转基因小麦中氨基酸含量的分析及比较[J].中国食物与营养,2017,23(4):56-58,3.基金项目
转基因生物新品种培育国家科技重大专项课题“转基因生物的食用和饲用安全评价技术”(项目编号:2014ZX08011-005、 2016ZX08011-005). （项目编号:2014ZX08011-005、 2016ZX08011-005）
