食品科学2017，Vol.38Issue(3)：53-58,6.DOI:10.7506/spkx1002-6630-201703009
高压脉冲电场对抗氧化肽荧光特性的影响
Effect of Pulsed Electric Field (PEF) on Fluorescence Characteristics of Antioxidant Peptides
摘要
Abstract
Two antioxidant peptides obtained in our previous work,Gln-Trp-Phe-Met (QWFM,652.78) and Lys-Trp-Phe-Met (KWFM,610.78),were used to explore the effect of pulsed electric field (PEF) on their fluorescence intensity.The results showed that the fluorescence intensity of both antioxidant peptides,which differed by N-terminal amino acid residues,especially QWFM,was changed after PEF treatment.The most significant change in fluorescence intensity of QWFM was observed at electric pulse frequency of 1 800 Hz and field intensity of 15 kV/cm.The fluorescence intensity of antioxidant peptides decreased as time elapsed after PEF treatment.The circular dichroism (CD) and nuclear magnetic resonance (NMR) spectra showed changed hydrogen bonds maintaining the β-sheet in QWFM and thus reduced β-sheet content.These results suggested that the chemical structure of antioxidant peptides and consequently their properties could be changed by PEF,which will provide a theoretical foundation for further exploitation and utilization of antioxidant peptides.关键词
高压脉冲电场/抗氧化肽/荧光强度/化学结构Key words
pulsed electric field (PEF)/antioxidant peptide/fluorescence intensity/chemical structure分类
轻工纺织引用本文复制引用
王莹,刘静波,邢杰,李幸芳,殷涌光..高压脉冲电场对抗氧化肽荧光特性的影响[J].食品科学,2017,38(3):53-58,6.基金项目
国家高技术研究发展计划(863计划)项目(2013AA102206-5) （863计划）
