食品科学2017，Vol.38Issue(3)：59-63,5.DOI:10.7506/spkx1002-6630-201703010
松仁谷蛋白抗氧化肽的分离纯化及一级结构鉴定
Isolation, Purification and Structural Analysis of Antioxidant Peptides Derived from Gluten in Pinus koraiensis Sieb.et Zucc Nut Kernels
摘要
Abstract
In this work,the enzymatic hydrolysate of pine nut gluten produced with alkaline protease was isolated and purified by ultrafiltration,Sephadex G-25,Sephadex G-15 and reversed-phase high-performance liquid chromatography (RP-HPLC).The structural characteristics of the purified fractions were identified by Q Exactive hybrid quadrupole-Orbitrap mass spectrometry,and de novo sequencing was performed based on the mass spectral data.Two antioxidant peptides were obtained with amino acid sequence of Glu-Leu-Leu-Lys-Leu-His (ELLKLH) and Glu-Lys-Asp-Phe-His-Leu (EKDFHL),respectively.After solid-phase synthesis,the purity of the peptides was 99.67％ and 95.15％,respectively.The results of antioxidant activities showed that the DPPH free radical scavenging rates of ELLKLH and EKDFHL at a concentration of 1 000 μmol/mL were 38.26％ and 29.54％,respectively,and the iron ion chelating rates were 56.44％ and 48.66％,respectively.关键词
松仁谷蛋白/分离纯化/结构鉴定Key words
pine nut glutenin/isolation and purification/structural identification分类
轻工纺织引用本文复制引用
毛晶,王鹏,王祖浩,方丽,王辑,闵伟红..松仁谷蛋白抗氧化肽的分离纯化及一级结构鉴定[J].食品科学,2017,38(3):59-63,5.基金项目
国家高技术研究发展计划(863计划)项目(2013AA102206) （863计划）
