食品科学2017，Vol.38Issue(3)：155-163,9.DOI:10.7506/spkx1002-6630-201703026
嗜酸乳杆菌胞外蛋白调控MAPK和PI3K-AKT信号途径关键蛋白活化水平
Extracellular Proteins from Lactobacillus acidophilus Regulate the Activation of Critical Proteins Involved in the MAPK and PI3K-AKT Signaling Pathways
摘要
Abstract
Purpose:To explore the role and molecular mechanism of proteins secreted by L.acidophilus CICC6005 in promoting intestinal health and to reveal that this topic is well worth further investigation.Methods:This experiment determined extracellular proteins secreted by L.acidophilus CICC6005 to inhibit the proliferation of HT-29 cells and further explored the pathway by which the extracellular proteins 67 and 37 ku inhibited tumor proliferation.In the present study,HT-29 cells were used as target cells to evaluate the expression levels of the critical target protein p38,c-Jun N-terminal kinase (JNK),c-Jun N-terminal kinase (ERK),phosphorylated p38 (p-p38),phosphorylated c-Jun N-terminal kinase (p-JNK),phosphorylated extracellular signal-regulated kinase (p-ERK),phosphorylated protein kinase B (p-AKT),phosphatidylinositol-3-kinase (PI3K) in the mitogen activated protein kinase (MAPK) and (phosphatidylinositol 3-kinase-protein kinase B) PI3K-AKT signaling pathways through Western blotting in order to explore the molecular mechanism by which that the extracellular proteins from L.acidophilus CICC6005 regulated the proliferation of colon cancer cells.Results:Exposure of HT-29 cells to different concentrations of 67 and 37 ku extracellular proteins from L.acidophilus CICC6005 could significantly inhibit the expression of p-ERK1/2,p-p38,p-AKT and PI3K in a dosedependent manner for both signaling pathways but not the expression of p-JNK,ERK1/2,p38 and JNK.Conclusion:The extracellular proteins 37 and 67 ku from L.acidophilus CICC6005 could inhibit the proliferation of HT-29 cells,and the mechanism might be related to the activation level of the critical proteins involved in the MAPK and PI3K-AKT pathways.Consumption of extracellular proteins from L.acidophilus could help to maintain intestinal health.关键词
嗜酸乳杆菌CICC6005/胞外蛋白/HT-29结肠癌细胞/丝裂原活化蛋白激酶(MAPK)/磷脂酰肌醇-3激酶-丝氨酸-苏氨酸蛋白激酶(PI3K-AKT)Key words
Lactobacillus acidophilus CICC6005/extracellular proteins/colon cancer cell line HT-29/mitogen activated protein kinase (MAPK)/phosphatidylinositol-3-kinase/protein kinase B (PI3K-AKT)分类
轻工纺织引用本文复制引用
王泳,贾彦,任效东,明珠,江岩,赵培,庞广昌,阎亚丽,陈庆森..嗜酸乳杆菌胞外蛋白调控MAPK和PI3K-AKT信号途径关键蛋白活化水平[J].食品科学,2017,38(3):155-163,9.基金项目
国家自然科学基金面上项目(31071522) （31071522）
天津商业大学国家基金培育项目(160120) （160120）
