食品科学2017，Vol.38Issue(3)：170-175,6.DOI:10.7506/spkx1002-6630-201703028
黑灵芝多糖对丙烯酰胺诱导小肠上皮细胞氧化损伤的保护作用
Protective Effect of Ganoderma atrum Polysaccharides on Oxidative Damage Induced by Acrylamide in IEC-6 Cells
摘要
Abstract
Objective:To investigate the protective effect of Ganoderma atrum polysaccharides (GAP) against oxidative injury induced by acrylamide (AA) in intestinal epithelial cells (IEC)-6.Methods:The MTT assay was used to evaluate the proliferation of IEC-6.After cultivation,the cells and culture medium were collected for determining the activities of lactate dehydrogenase (LDH),malondialdelyde (MDA),superoxide dismutase (SOD) and reduced glutathione peroxidase (GSH-Px).Results:GAP had no toxic effect on the cultured IEC-6 at the tested concentrations.Compared with the AA-treated group,GAP significantly attenuated AA-induced oxidative damage and increased cell viability by decreasing LDH activity and MDA content,and increasing SOD and GSH-Px activities in IEC-6 line.Conclusion:These results suggested that GAP showed a protective effect against AA-induced oxidative damage in IEC-6 through inhibiting lipid peroxidation,and increasing antioxidant system activity.关键词
黑灵芝多糖/丙烯酰胺/IEC-6/氧化损伤Key words
Ganoderma atrum polysaccharides/acrylamide/IEC-6/oxidative damage分类
轻工纺织引用本文复制引用
张路路,石婷,朱梦婷,陈奕..黑灵芝多糖对丙烯酰胺诱导小肠上皮细胞氧化损伤的保护作用[J].食品科学,2017,38(3):170-175,6.基金项目
国家自然科学基金地区科学基金项目(21467016) （21467016）
全国优秀博士学位论文作者专项资金项目(201357) （201357）
江西省青年科学家培养对象计划项目(20142BCB23005) （20142BCB23005）
