食品科学2017，Vol.38Issue(3)：195-200,6.DOI:10.7506/spkx1002-6630-201703032
阿魏酸拮抗PM2.5对A549细胞线粒体的损伤作用
Protective Effect of Ferulic Acid on PM2.5-Induced Mitochondrial Damage in A549 Cells
摘要
Abstract
In this study,we examined whether ferulic acid (FA) could protect against PM2.5-induced mitochondrial damage in A549 cells and explored the underlying mechanism.The cells were treated with PM2.5 (240 μg/mL) with and without the addition of 80 mmol/L FA.The contents of reactive oxygen species (ROS) were determined by flow cytometry using MitoSOX Red specific probe.Apoptosis was detected by flow cytometry with Annexin-V/PI method.Mitochondrial membrane potential was detected by flow cytometry with JC-1 staining.The activity of mitochondrial membrane channel pore was tested by mitochondrial permeability transition pore (mPTP) kit.The expression of caspase-3,caspase-9,manganese superoxide dismutase (MnSOD),and peroxisome proliferators-activated receptor-γ-coactivator-1α (FGC-1α) were detected by Western blotting.The results showed that,compared with the blank control group,the level of ROS and the expression of caspase-3 and caspase-9 in mitochondria,as well as the apoptosis rate ofA549 cells induced by PM2.5 were increased.The membrane potential and the activity of mPTP in mitochondria ofA549 cells induced by PM2.5 were decreased as well as the expression of PGC-1α and MnSOD.The pre-treatment of FA could prevent the increased mitochondrial ROS,apoptosis rate and expression of caspase-3 and caspase-9,and the decreased mitochondrial membrane potential,mPTP activity,and expression of PGC-1α and MnSOD caused by PM2.5.Therefore,FA had a significant protective effect on PM2.5-induced mitochondrial damage in A549 cells.关键词
阿魏酸/PM2.5/A549细胞/线粒体/损伤/保护作用Key words
ferulic acid/PM2.5/A549 cells/mitochondria/damage/protective effect分类
医药卫生引用本文复制引用
劳文艳,毕婷婷,周艳丽,陈世杰,赵晓红,刁屹..阿魏酸拮抗PM2.5对A549细胞线粒体的损伤作用[J].食品科学,2017,38(3):195-200,6.基金项目
中国营养学会营养科研基金-帝斯曼专项科研基金项目(2015-030 (CNS-DSM)) （2015-030 (CNS-DSM）
北京联合大学生物活性物质与功能食品北京市重点实验室开放课题(Zk70201501) （Zk70201501）
