食品科学2017，Vol.38Issue(4)：26-31,6.DOI:10.7506/spkx1002-6630-201704005
内源性氧化胁迫促进酿酒酵母合成谷胱甘肽的潜在机制分析
Elucidation of the Underlying Mechanism by Which Endogenous Oxidative Stress Promotes Glutathione Synthesis of Saccharomyces cerevisiae
摘要
Abstract
The potential mechanism for glutathione oversynthesis in the Saccharomyces cerevisiae mutant Y518 was researched using transcriptome analysis combined with physiological and biochemical characteristics.The results indicated that the rate-limiting enzyme of glutathione synthesis,antioxidant enzymes activities and the expression levels of their encoding genes,and the contents of hydrogen peroxide and nicotinamide adenine dinucleotide phosphate (NADPH) were significantly increased in the mutant whereas pyruvatekinase activity,the contents ofpyruvate,citrate and succinate were markedly decreased.Besides,the expression levels of genes involved in the citrate cycle were significantly down-regulated while those involved in the pentose phosphate pathway were significantly up-regulated.Therefore,tunder endogenous oxidative Stress,the mutant might strengthen the synthesis of glutathione by adjusting the activities of rate-limiting enzymes of glutathione synthesis to defend against oxidative stress together with the antioxidant enzymes.Meanwhile,weakened pyruvatekinase activity decreased pyruvate generation,which led to declined citrate cycle flux and increased NADPH production by the pentose phosphate pathway and consequently provided appropriate reducing power for glutathione biosynthesis.关键词
谷胱甘肽/氧化胁迫/酿酒酵母/还原型辅酶ⅡKey words
glutathione/oxidative stress/Saccharomyces cerevisiae/NADPH分类
生物科学引用本文复制引用
王立梅,任清华,郑丽雪,孙姜,齐斌,朱益波..内源性氧化胁迫促进酿酒酵母合成谷胱甘肽的潜在机制分析[J].食品科学,2017,38(4):26-31,6.基金项目
国家自然科学基金面上项目(31171758) （31171758）
