食品科学2017，Vol.38Issue(4)：113-117,5.DOI:10.7506/spkx1002-6630-201704019
单增李斯特菌srtA基因敲除菌株的构建及其生物学特性
Construction and Biological Properties of srtA Gene Knockout Mutant Strains of Listeria monocytogenes
摘要
Abstract
Listeria monocytogenes is a common foodborne pathogen.Cell wall-anchored surface proteins are closely related to the pathogenicity and biofilm formation of the bacterium.The srtA gene is a key factor that mediates the membrane anchoring of surface proteins,and as an important virulence gene of L.monocytogenes it can covalently bind surface proteins to the cell wall by recognizing specific peptide sequences.For a better understanding of the function of srtA and its role in bacterial virulence regulation,we knocked out the srtA gene in L.monocytogenes by homologous recombination in this study,and evaluated the biological properties of the mutant strain.Based on its growth curve examination,we found that the growth rate of the mutant strain was slower than that of the wild-type one.Furthermore,the invasion efficiency of the mutant strain in glioma U251 cells was lower than that of the wild-type one.These results indicate that srtA may play an important role in regulating the invasiveness of L.monocytogenes,which will provide a theoretical basis for further understanding of the regulatory mechanisms of virulence genes in L.monocytogenes and biofilm formation.关键词
单增李斯特菌/srtA基因/同源重组/生长曲线/侵袭Key words
Listeria monocytogenes/srtA gene/homologous recombination/growth curve/invasion分类
轻工纺织引用本文复制引用
李森,热依汉古丽·麦麦提力..单增李斯特菌srtA基因敲除菌株的构建及其生物学特性[J].食品科学,2017,38(4):113-117,5.基金项目
上海理工大学博士启动基金资助项目(1000308001) （1000308001）
上海市高校青年教师资助计划项目(10-15-308-802) （10-15-308-802）
