食品科学2017，Vol.38Issue(4)：170-176,7.DOI:10.7506/spkx1002-6630-201704027
全自动氨基酸分析仪法测定4种贝类肌肉中谷胱甘肽和游离氨基酸含量
Determination of Glutathione and Free Amino Acids in Muscles of Four Shellfish Species by Automatic Amino Acid Analyzer
摘要
Abstract
An analytical method was established for the simultaneous determination of glutathione and free amino acids in shellfish using a fully automatic amino acid analyzer.The extraction of the analytes was carried out using 0.02 mol/L hydrochloric acid solution as the extraction solvent followed by protein precipitation with 5％ sulfonic acid solution.The buffer composition and the elution procedure were optimized at the same time.The linear range,limit of detection (LOD),precision and recovery rate of the method were determined.The results showed that good separation of glutathione and free amino acids in shellfish was achieved by this method with a good linear relationship.The correlation coefficients of the calibration curves were between 0.999 1 and 0.999 9.The minimum LODs at a signal to noise ratio (RsN) of 3 ranged from 0.07 to 0.27 μmol/L.The average recovery rates of spiked samples were between 86.40％ and 102.42％.The relative standard deviations (RSDs) of intra-day and inter-day assays were 0.31％-).73％ and 1.14％-2.60％,respectively.Free amino acids were abundant in muscle tissue of four shellfish species,and their total free amino acid contents in decreasing order were clam (1 396.39 mg/100 g) ＞ Cyclina sinensis (1 016.04 mg/100 g) ＞ razor clam (911.15 mg/100 g) ＞ oyster (287.01 mg/100 g).The main free amino acids were taurine,glutamic acid,alanine,and arginine.The glutathione contents of Cyclina sinensis,clam,razor clam and oyster were 103.20,82.53,61.77,and 33.37 mg/100 g,respectively.This method is suitable for the determination of glutathione and free amino acids in shellfish.关键词
贝类肌肉/谷胱甘肽/游离氨基酸/全自动氨基酸分析仪法Key words
shellfish muscle/glutathione/free amino acids/automatic amino acid analyzer分类
轻工纺织引用本文复制引用
张苏平,邱伟强,卢祺,陈舜胜..全自动氨基酸分析仪法测定4种贝类肌肉中谷胱甘肽和游离氨基酸含量[J].食品科学,2017,38(4):170-176,7.基金项目
国家自然科学基金面上项目(31471685) （31471685）
上海海洋大学校科技发展专项(A2-0203-00-100208) （A2-0203-00-100208）
上海海洋大学校博士启动基金项目(A2-0203-00-100340) （A2-0203-00-100340）
