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响应面试验优化黏质沙雷氏菌利用菜籽饼粕产灵菌红素工艺

陈新颜 操丽丽 杨培周 王康林 章华 操新民 郑志 姜绍通

食品科学2017,Vol.38Issue(4):231-236,6.
食品科学2017,Vol.38Issue(4):231-236,6.DOI:10.7506/spkx1002-6630-201704037

响应面试验优化黏质沙雷氏菌利用菜籽饼粕产灵菌红素工艺

Response Surface Optimization of Prodigiosin Production of Serratia marcescens Using Rapeseed Meal

陈新颜 1操丽丽 1杨培周 1王康林 2章华 1操新民 1郑志 1姜绍通1

作者信息

  • 1. 合肥工业大学食品科学与工程学院,安徽省农产品加工精深省级实验室,安徽合肥 230009
  • 2. 平光制药股份有限公司,安徽合肥 231131
  • 折叠

摘要

Abstract

A culture medium containing rapeseed meal was designed for prodigiosin production by Serratia marcescens.Optimizations of culture medium composition and fermentation conditions by response surface method were performed for improved production of prodigiosin.The results indicated that the optimal medium composition was composed of corn powder 10 g/L,rapeseed meal 21.7 g/L,inoculum concentration 5.5%,and zinc sulfate 0.05 g/L at an initial pH of 5.8.When the strain was cultured for 24 h in 80 mL of the medium contained in a 250 mL flask with shaking at 200 r/min,the fermented broth had the highest prodigiosin content of 11.56 g/L.This study could provide a basis for the microbial production of prodigiosin from high-temperature rapeseed meal.

关键词

黏质沙雷氏菌/灵菌红素/菜籽饼粕/响应面优化/发酵

Key words

Serratia marcescens/prodigiosin/rapeseed meal/response surface optimization/fermentation

分类

轻工纺织

引用本文复制引用

陈新颜,操丽丽,杨培周,王康林,章华,操新民,郑志,姜绍通..响应面试验优化黏质沙雷氏菌利用菜籽饼粕产灵菌红素工艺[J].食品科学,2017,38(4):231-236,6.

基金项目

合肥工业大学大学生创新创业训练项目(201510359056) (201510359056)

安徽省科技攻关项目(1604a0702001) (1604a0702001)

食品科学

OA北大核心CSCDCSTPCD

1002-6630

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