食品科学2017,Vol.38Issue(4):231-236,6.DOI:10.7506/spkx1002-6630-201704037
响应面试验优化黏质沙雷氏菌利用菜籽饼粕产灵菌红素工艺
Response Surface Optimization of Prodigiosin Production of Serratia marcescens Using Rapeseed Meal
摘要
Abstract
A culture medium containing rapeseed meal was designed for prodigiosin production by Serratia marcescens.Optimizations of culture medium composition and fermentation conditions by response surface method were performed for improved production of prodigiosin.The results indicated that the optimal medium composition was composed of corn powder 10 g/L,rapeseed meal 21.7 g/L,inoculum concentration 5.5%,and zinc sulfate 0.05 g/L at an initial pH of 5.8.When the strain was cultured for 24 h in 80 mL of the medium contained in a 250 mL flask with shaking at 200 r/min,the fermented broth had the highest prodigiosin content of 11.56 g/L.This study could provide a basis for the microbial production of prodigiosin from high-temperature rapeseed meal.关键词
黏质沙雷氏菌/灵菌红素/菜籽饼粕/响应面优化/发酵Key words
Serratia marcescens/prodigiosin/rapeseed meal/response surface optimization/fermentation分类
轻工纺织引用本文复制引用
陈新颜,操丽丽,杨培周,王康林,章华,操新民,郑志,姜绍通..响应面试验优化黏质沙雷氏菌利用菜籽饼粕产灵菌红素工艺[J].食品科学,2017,38(4):231-236,6.基金项目
合肥工业大学大学生创新创业训练项目(201510359056) (201510359056)
安徽省科技攻关项目(1604a0702001) (1604a0702001)