食品科学2017，Vol.38Issue(4)：260-264,5.DOI:10.7506/spkx1002-6630-201704042
河豚毒素DNA适配子核心区域的识别
Identification of Core Region of DNA Aptamers against Tetrodotoxin
摘要
Abstract
The secondary structures of DNA aptamers A3 and G10 against tetrodotoxin were analyzed using DNA folding form online software.Further,digoxin labeled 5'end sequences of four regions in each aptamer were separately synthesized.Tetrodotoxin was fixed by acrylic acid-epoxy ethane beads.The core regions of these aptamers were identified using digoxin-anti digoxin alkaline phosphatase system to detect the affinity of these various regions with tetrodotoxin,after determining the appropriate dilution ratio of anti-digoxin alkaline phosphatase.The results showed that the suitable working concentration of anti-digoxin alkaline phosphatase was 2 × 104 times dilution.The core region of aptamer A3 was A3-2 area which had the strongest affinity with tetrodotoxin among the four regions,including 47 nucleotides,while the core region of aptamer G10 was G10-4 area,including 56 nucleotides.关键词
河豚毒素/适配子/核心区域/亲和力Key words
tetrodotoxin/aptamer/core region/affinity分类
生物科学引用本文复制引用
邵碧英,陈文炳,刘正才,缪婷玉,彭娟,杨方..河豚毒素DNA适配子核心区域的识别[J].食品科学,2017,38(4):260-264,5.基金项目
国家质检总局科技计划项目(2014IK104) （2014IK104）
